Journal: Frontiers in pharmacology

Article Title: Ginsenoside Rb1 Lessens Gastric Precancerous Lesions by Interfering With β-Catenin/TCF4 Interaction.

doi: 10.3389/fphar.2021.682713

Figure Lengend Snippet: FIGURE 6 | GRb1 inhibits hyper-proliferation of epithelial cells and accelerates apoptosis in GPL model rats. Representative images showing (A) PCNA expression and (C) Ki-67 expression as well as (E) TUNEL apoptotic cells in gastric tissue sections from each group (magnification ×100 and ×400). Semi-quantitative analysis of (B) PCNA protein expression levels and (D) Ki-67 protein expression levels as well as (F) TUNEL apoptosis ratio in each group (n 10). *p < 0.05 and **p < 0.01 vs. Normal group. #p < 0.05 and ##p < 0.01 vs. Model group. Data are presented as mean ± SEM. Abbreviations: GRb1, ginsenoside Rb1; GPL, gastric precancerous lesions; PCNA, proliferating cell nuclear antigen; TUNEL, TdT-mediated dUTP nick-end labeling; SEM, standard error of mean.

Article Snippet: Ki67 antibody was supplied by Novus Biologicals, LLC, United States (cat. no. NB500-170).

Techniques: Expressing, TUNEL Assay, End Labeling

Journal: Cell death discovery

Article Title: NeuroD4 converts glioblastoma cells into neuron-like cells through the SLC7A11-GSH-GPX4 antioxidant axis.

doi: 10.1038/s41420-023-01595-8

Figure Lengend Snippet: Fig. 3 NeuroD4-induced neuronal reprogramming suppresses the proliferative marker EdU and Ki67 in glioblastoma cells. A The experimental design for labeling EdU in glioblastoma cells. B–E EdU detection of U251 and KNS89 cells at 14 dpi and quantitative analysis of EdU+ (9 random fields from triplicate samples were captured for quantification). F–I Immunocytochemical analysis of the Ki67 marker in U251 and KNS89 cells at 14 dpi and quantitative analysis of Ki67+ (9 random fields from triplicate samples were captured for quantification). J, K Time-course cell counting at 0, 1, 3, 5, 7, 14, and 21 dpi. The data are presented as mean ± SD. ***P < 0.001. Dpi: days post infection; GM glioblastoma cell medium, NM neuronal induction medium. Scale: 100 µm.

Article Snippet: After that, samples were incubated at 4 °C overnight with primary antibodies (TUJ1 (ab78078), MAP2 (ab96378), Ki67 (ab15580) and GFAP (ab 68428) from Abcam, USA, SOX2 (11064-1-AP), SOX10 (10422- 1-AP) and OLIG2 (13999-1-AP) from Proteintech, China) and then at 37 °C for 1 h with secondary antibodies (ab150080/ab150116, Abcam, USA, 1:1000).

Techniques: Marker, Labeling, Cell Counting, Infection

Journal: Cell death discovery

Article Title: NeuroD4 converts glioblastoma cells into neuron-like cells through the SLC7A11-GSH-GPX4 antioxidant axis.

doi: 10.1038/s41420-023-01595-8

Figure Lengend Snippet: Fig. 5 The xenografts derived from NeuroD4 overexpressing U87 cells shrink significantly. A The experimental protocol for orthotopic cell transplantation. B, C In vivo bioluminescent images and the quantification of U87-derived xenografts (n = 5). D Post-imaging Kaplan-Meier survival analysis of transplanted mice (n = 10, P < 0.001 using log-rank test). E, F Immunohisto fluorescence analysis of tumor size in mice implanted with GFP and GFP+NeuroD4 virus infected U87 cells 28 days after transplantation (n = 6). Tumor mass (outlined by dashed lines) was quantified based on the area occupying the ipsilateral brain. G–I Ki67 and TUJ1 markers of NeuroD4 overexpressing U87 cells in xenografts 28 days after implantation. Forty-five random fields from a series of every tenth coronal brain section of six nude mice were collected for quantification in Immunohisto fluorescence analysis. The data are presented as mean ± SD. ***P < 0.001 by student’s t-test and One-way analysis of variance (ANOVA). NS: no significance. ND not detected. GM glioblastoma cell medium. Scale: 100 µm.

Article Snippet: After that, samples were incubated at 4 °C overnight with primary antibodies (TUJ1 (ab78078), MAP2 (ab96378), Ki67 (ab15580) and GFAP (ab 68428) from Abcam, USA, SOX2 (11064-1-AP), SOX10 (10422- 1-AP) and OLIG2 (13999-1-AP) from Proteintech, China) and then at 37 °C for 1 h with secondary antibodies (ab150080/ab150116, Abcam, USA, 1:1000).

Techniques: Derivative Assay, Transplantation Assay, In Vivo, Imaging, Virus, Infection

Journal: Cell death discovery

Article Title: NeuroD4 converts glioblastoma cells into neuron-like cells through the SLC7A11-GSH-GPX4 antioxidant axis.

doi: 10.1038/s41420-023-01595-8

Figure Lengend Snippet: Fig. 7 SLC7A11-GSH-GPX4 antioxidant axis is a key in NeuroD4-mediated neuronal reprogramming. A, B The fluorescence intensity of Reactive oxygen species (ROS) was detected by flow cytometry at 5 dpi. C–E Western blot analysis of SLC7A11 and GPX4 protein in U251 cells infected with GFP and GFP+NeuroD4 virus at 3 and 7 dpi. (n = 3). F–H Ki67 and TUJ1 staining revealed the reprogramming efficiency of particular lentiviruses-infected U251 cells at 14 dpi (9 random fields from triplicate samples were captured for quantification). I Pathways involved in NeuroD4-induced reprogramming. GSR glutathione-disulfide reductase. GSSG glutathione oxidized. The data are presented as mean ± SD. *P < 0.01, **P < 0.005, ***P < 0.001 by student’s t-test. Dpi days post infection. NS no significance. ND not detected. Scale: 100 µm.

Article Snippet: After that, samples were incubated at 4 °C overnight with primary antibodies (TUJ1 (ab78078), MAP2 (ab96378), Ki67 (ab15580) and GFAP (ab 68428) from Abcam, USA, SOX2 (11064-1-AP), SOX10 (10422- 1-AP) and OLIG2 (13999-1-AP) from Proteintech, China) and then at 37 °C for 1 h with secondary antibodies (ab150080/ab150116, Abcam, USA, 1:1000).

Techniques: Cytometry, Western Blot, Infection, Virus, Staining

Journal: Experimental and Therapeutic Medicine

Article Title: Evaluation of rat C6 malignant glioma using spectral computed tomography

doi: 10.3892/etm.2017.4613

Figure Lengend Snippet: Comparison of spectral CT-derived parameters (at 65 keV) and Ki67 expression (immunohistochemistry) between normal brain tissue and various tumor regions in a rat C6 malignant glioma model.

Article Snippet: The sample was dehydrated, embedded in paraffin, sectioned at 3–4 μm, and either stained with hematoxylin and eosin (HE) or immunostained for Ki67 (a marker of cell proliferation) using an anti-Ki67 primary antibody (1:180; Biorbyt, Wuhan, China) and an EnVision system in accordance with the manufacturer's instructions (Dako, Agilent Technologies, Santa Clara, CA, USA).

Techniques: Comparison, Expressing, Immunohistochemistry

Journal: Experimental and Therapeutic Medicine

Article Title: Evaluation of rat C6 malignant glioma using spectral computed tomography

doi: 10.3892/etm.2017.4613

Figure Lengend Snippet: Histopathology of a rat C6 glioma. (A) Representative section of a tumor stained with hematoxylin and eosin (×100). (B) Representative section showing solid tumor and peritumoral region, stained with hematoxylin and eosin (×200). (C) Representative section of liquefactive necrosis within a tumor, immunostained for Ki67 (×200). (D) Representative section showing solid tumor and peritumoral region, immunostained for Ki67 (×400). Green arrows indicate liquefactive necrosis; red arrows indicate solid tumor; yellow arrows indicate an area of tumor cell infiltration; and blue arrows indicate neovascularization. (Images are representative of n =13).

Article Snippet: The sample was dehydrated, embedded in paraffin, sectioned at 3–4 μm, and either stained with hematoxylin and eosin (HE) or immunostained for Ki67 (a marker of cell proliferation) using an anti-Ki67 primary antibody (1:180; Biorbyt, Wuhan, China) and an EnVision system in accordance with the manufacturer's instructions (Dako, Agilent Technologies, Santa Clara, CA, USA).

Techniques: Histopathology, Staining

Journal: Experimental and Therapeutic Medicine

Article Title: Evaluation of rat C6 malignant glioma using spectral computed tomography

doi: 10.3892/etm.2017.4613

Figure Lengend Snippet: Pearson correlation analysis of the relation between Ki67 expression (immunohistochemistry) and spectral CT-derived parameters measured in normal brain tissue and various tumor regions (liquefactive necrosis, solid tumor, peripheral tumor).

Article Snippet: The sample was dehydrated, embedded in paraffin, sectioned at 3–4 μm, and either stained with hematoxylin and eosin (HE) or immunostained for Ki67 (a marker of cell proliferation) using an anti-Ki67 primary antibody (1:180; Biorbyt, Wuhan, China) and an EnVision system in accordance with the manufacturer's instructions (Dako, Agilent Technologies, Santa Clara, CA, USA).

Techniques: Expressing, Immunohistochemistry, Standard Deviation

Journal: Experimental and Therapeutic Medicine

Article Title: Evaluation of rat C6 malignant glioma using spectral computed tomography

doi: 10.3892/etm.2017.4613

Figure Lengend Snippet: Analysis of the correlations between Ki67 expression and parameters measured using spectral CT. (A) Pearson correlation analysis of the association between Ki67 expression and the slope of the spectral curve. (B) Pearson correlation analysis of the association between Ki67 expression and the CT value at 65 keV (HU).

Article Snippet: The sample was dehydrated, embedded in paraffin, sectioned at 3–4 μm, and either stained with hematoxylin and eosin (HE) or immunostained for Ki67 (a marker of cell proliferation) using an anti-Ki67 primary antibody (1:180; Biorbyt, Wuhan, China) and an EnVision system in accordance with the manufacturer's instructions (Dako, Agilent Technologies, Santa Clara, CA, USA).

Techniques: Expressing

Journal: Molecules

Article Title: Withanolides, Extracted from Datura Metel L. Inhibit Keratinocyte Proliferation and Imiquimod-Induced Psoriasis-Like Dermatitis via the STAT3/P38/ERK1/2 Pathway

doi: 10.3390/molecules24142596

Figure Lengend Snippet: Figure 4. The psoriasis-like skin sections were strained by immunohistochemistry to evaluate Ki67 in five groups. (A) Control group. (B) IMQ-treated group. (C) Withanolides 500 mg/kg group. (D) Withanolides 250 mg/kg group. (E) MTX-treated group. (F) Ki67 positive cell/field (IF ×400). *** p < 0.001 vs. IMQ-treated group, ### p < 0.001 vs. control group.

Article Snippet: CD3E, F4/80, Ki67, P38, p-P38 (Tyr323) and GAPDH primary antibodies were acquired from Bioss Biotechnology Co. Ltd. (Beijing, China).

Techniques: Immunohistochemistry, Control